Comparative Pharmacokinetic Studies of Cetirizine Tablets in Healthy Human Volunteers

An Optimized LC-MS condition was proposed for the estimation in human plasma. Cetrizine had been chromatographed on a C18 column with a mobile phase Water and Methanol in the ratio of 60:40v/v. the mobile phase was pumped in the flow rate of 0.5ml/min. Erythromycin was used as an internal standard and eluents were monitored at 749 nm. The data presented in this report were validated for the estimation of cetrizine in human plasma over a concentration range of 10.0-600.0 ng/ml. The precision and accuracy are very much within the prescribed limit in this concentration ranges. The values obtained from the system suitability studies demonstrate the suitability of the system for the analysis of Cetrizine in plasma. Limit of Detection of the method was 3.0ng/ml and Limit of Quantization was 10.0ng/ml which shows that the developed method has adequate sensitivity and also more than 50 samples can be processed at a time without affecting the assay values. The method developed was accurate, accordingly to the requirement of LCMS which can be use4d for the estimation of Cetrizine in plasma samples.